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Egyptian Liver Journal
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Correlation of non-alcoholic fatty liver disease and gut microflora: clinical reports and treatment options

Egyptian Liver Journal volume 14, Article number: 21 (2024) Cite this article

Abstract

A prevalent liver condition called non-alcoholic fatty liver disease (NAFLD) may progress into non-alcoholic steatohepatitis (NASH) and cause life-threatening complications like cirrhosis and liver cancer. The development and progression of NAFLD has been linked to the make-up and functioning of the gut microflora. This article reviews the clinical studies reported to investigate the connection between changes in the gut microbiota and metabolic markers in NAFLD patients. According to the study findings, dysbiosis of the gut microflora were observed in NAFLD patients, which are manifested by variations in the proportions of particular bacterial species. These changes are linked to fibrosis, liver inflammation, and metabolic abnormalities. The article also discusses various treatments targeting the gut microbiota, including dietary modifications, exercise, prebiotics, probiotics, synbiotics, antibiotics, and fecal microbiota transplantation. These therapies are intended to enhance NAFLD outcomes and reestablish the healthy gut microflora. While some studies have shown promising results, further research is needed to establish the optimal approaches, long-term safety, and efficacy of these treatments for NAFLD.

Introduction

Non-alcoholic fatty liver disease (NAFLD) is characterized by the accumulation of fat in liver cells, unrelated to alcohol consumption. When the fat level in the liver exceeds 5–10% of its weight, inflammation occurs. If NAFLD progresses, it can lead to non-alcoholic steatohepatitis (NASH), which increases the risk of liver cancer, fibrosis, cirrhosis, and liver failure. Approximately one in five individuals with NAFLD eventually develops NASH, and if left untreated, NAFLD can increase the risk of liver cancer [1, 2]. The etiology of NAFLD is complex and influenced by biological, environmental, and lifestyle factors. Recent research has highlighted the crucial role of gut dysbiosis, an imbalance in the gut microbiota composition and function, in the development of NAFLD. The disruption of the symbiotic relationship between the host and gut microorganisms contributes to NAFLD pathogenesis, emphasizing the significance of the gut–liver axis [3,4,5].

Gut dysbiosis is characterized by a variation in the makeup and functioning of the gut microflora. Dysbiosis increases the formation of microbial metabolites, changes the way by which gut barrier works, and stirs up immunological responses, all of which have been linked to inflammation associated with NAFLD (Fig. 1). Dysbiosis-induced inflammation can cause the liver to produce reactive oxygen species (ROS), which can harm the liver cells through oxidative stress. This oxidative stress exacerbates liver inflammation further and hastens NAFLD progression [6,7,8].

Fig. 1
figure 1

Gut dysbiosis and NAFLD

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In our prior review, we studied the complex connection between gut dysbiosis and NAFLD [9]. We explored the underlying mechanisms that intricately link the liver, gut, and systemic metabolism. These mechanisms encompass alterations in gut permeability, inflammation, immunological dysregulation, changes in bile acid metabolism, and the impact of microbiota-derived metabolites. Through a comprehensive analysis of the existing research and experimental studies documented in various literature databases, we elucidated the pathways connecting gut dysbiosis to the initiation and progression of NAFLD. Further in this article, we provide a comprehensive review of clinical investigations that have examined the relationship between alterations in the gut microflora and metabolic indicators in individuals diagnosed with NAFLD. Additionally, the paper offers an in-depth discussion of therapeutic interventions aimed at manipulating the gut microbiota, encompassing dietary adjustments, physical activity, prebiotics, probiotics, synbiotics, antibiotic treatments, and fecal microbiota transplantation.

Search strategy

A comprehensive search was conducted across five electronic databases: PubMed, Web of Science, Embase, ScienceDirect, and Google Scholar, covering the period from 2013 to August 2023. To investigate gut microbiota changes in relation to NAFLD, our search incorporated various keywords including “Cirrhosis,” “NAFLD,” “Liver Disease,” “NASH,” “Gut Microflora,” “Fecal Microbiome,” “Gut Dysbiosis,” “Steatohepatitis,” and “Simple Steatosis.” Our primary research emphasis focused on human studies concerning the diagnostic utility of fecal microbiota analysis in identifying NAFLD. To ensure the robustness of the data collected, we have included studies that employed analytical techniques like 16S rRNA gene sequencing, 16S rDNA sequencing, shotgun metagenomics sequencing, and quantitative real-time PCR.

In order to investigate therapeutic interventions, a comprehensive search strategy was employed, involving a diverse range of keywords such as “Probiotics,” “Lactobacillus,” “Bifidobacterium,” “Cirrhosis,” “NAFLD,” “Liver Disease,” “NASH,” and “Gut Microflora,” with a primary focus on human studies, particularly those exploring probiotic usage in NAFLD treatment.

Clinical studies on gut microbiota in NAFLD

Studies on humans have uncovered crucial information on the changes in gut microflora composition and functions related to NAFLD. Table 1 summarizes the clinical studies that have been documented correlating the emergence of variations in gut microflora among NAFLD patients.

Table 1 Evidence collected from human trials indicating associations among different bacterial species and metabolic markers in NAFLD patients
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HP-NASH histology proven non-alcoholic steatohepatitis, HC healthy controls, SS simple steatosis, PCR polymerase chain reaction, ALT alanine transaminase, AST aspartate transaminase, BP biopsy-proven, LC liver cirrhosis, LC-MS liquid chromatography-mass spectrometry, BMI body mass index

A series of studies investigated the role of gut microbiota and related factors in various liver conditions. Wong et al. [10] found that fecal dysbiosis significantly impacts individuals with NASH and that improvements in liver fat accumulation are associated with changes in the gut microflora. However, the study was limited to a relatively small sample population that included exclusively participants of Chinese origin, thereby limiting generalizability to a wider population. Mouzaki and coworkers [11] observed an inverse relationship between the occurrence of NASH and the quantity of Bacteroidetes in stool samples, suggesting the potential role of this microbiota in NAFLD progression. However, the study did not consider age as a confounding factor and used quantitative PCR, which may not identify new microbial species effectively.

Jiang et al. [12] analyzed 53 patients with NAFLD, reporting decreased levels of CD8+ and CD4+ T cells, elevated pro-inflammatory cytokines, and increased liver enzyme levels in these individuals. The homogenous focus of this study on Chinese volunteers however limits its correlation to a larger demographics. Boursier et al. [13] associated dysbiosis and metabolic changes in the gut microbiota with NAFLD severity, with specific microbial species linked to NASH and severe fibrosis, however again restricted to a small sample size, 57 NAFLD patients. Elevated plasma ALT levels and reduced abundance of several bacterial genera was linked by Wang et al. [14]. They also identified a negative correlation between Lactobacillus and various NAFLD markers. However, the study relied on ultrasound for NAFLD diagnosis, limiting disease severity assessment.

Shen et al. [15] suggested that decreased Prevotella levels could be detrimental in NAFLD, while higher levels of Blautia and E. Shigella might indicate NAFLD progression. However, the study’s limited BMI diversity requires further investigation of the impact of BMI on gut microbiota in NAFLD. Del Chierico and coworkers [16] in their study identified specific microbial markers for the onset and progression of NAFLD but emphasized the need for well-defined study groups for model validation. Hoyles et al. [17] connected microbiome changes and serum biomarkers to NAFLD, but their study exclusively included female volunteers, thereby raising concerns over its reproducibility in male counterparts. In the study carried out by Caussy et al. [18], the metabolites associated with hepatic fibrosis were identified, but exploration of the role of bile acid changes in NAFLD progression were not addressed. Li et al. [19] identified specific microbial taxa linked to obesity and NAFLD through metastatic analysis. However, the cross-sectional design of the study and lack of liver biopsy driven interpretation, limits the conclusions drawn. The association of changes in gut microbiota function with NAFLD were well studied by Schwimmer et al. [20]. However, this study failed to assess impact of dietary factors on disease progression.

Tsai et al. [21] suggested that shifts in bacterial composition impacts the development of NAFLD/NASH but did not correlate the LPS levels in NASH. Further, the study in specific ethnic population (Taiwan) is the major limitation. Daud and colleagues [22] reported reduced gut microbial diversity in NAFLD but involve a small sample population and lacked histopathological confirmation. Li et al. [23] proposed using specific microbial markers and metabolites for HCC diagnosis. However, this study again focused on a small sample population and did not consider disease prognosis.

In all the abovementioned studies among patients with NAFLD, a reduced diversity of gut bacteria is observed, linked to disease severity and progression. This decrease in microbial species is accompanied by an increase in potentially harmful bacteria, especially from the Proteobacteria family. This includes species like Escherichia coli, Lactobacillus, and Streptococcus, contributing to inflammation and increased gut permeability via endotoxin production. Beneficial anti-inflammatory bacteria like Bifidobacterium and F. prausnitzii are often diminished in NAFLD patients. The Firmicutes to Bacteroidetes (F/B) ratio, a biomarker of gut dysbiosis, tends to be higher in NAFLD, indicating an imbalance between these phyla. These shifts in gut microflora suggest a transition toward a more pro-inflammatory and less diverse microbial community in NAFLD, though specific profiles may vary among individuals and populations, highlighting the complexity of gut dysbiosis in the disease.

Limitations and challenges in studying impact of gut microbiota in NAFLD

Studying the correlation between gut dysbiosis and NAFLD underlines considerable challenges. The extensive diversity and individual variability of the gut microbiota hinder the identification of specific microbial patterns linked to NAFLD, influenced by genetics, lifestyle, and diet. Understanding whether changes in the microbiota cause NAFLD or if NAFLD leads to changes in the microbiota is complex due to the fact that there are interactions occurring in both directions. This complexity necessitates longitudinal studies, which track changes over time, in order to establish causal relationships. Often, studies investigating gut dysbiosis in NAFLD suffer from limited sample sizes and cohort diversity, with varied disease stages, ethnical, geographical, and cultural variations, posing obstacles to data interpretation and generalization. Research predominantly conducted in animal models may not fully mirror human NAFLD complexity, with translation hurdles arising from species differences and multifactorial disease nature. Inconsistent methodologies for sample collection, DNA extraction, and data analysis yield result variability, underlining the importance of reproducibility across research groups. The intricate interactions between microbial communities and host physiology supporting gut dysbiosis’ effect on NAFLD remain only partly understood, complicating the elucidation of precise mechanisms. Addressing these limitations necessitates well-designed, expansive studies featuring standardized approaches, integration of multi-omics data, and interdisciplinary collaborations, contributing to a more nuanced understanding of the gut-liver axis' role in NAFLD onset and progression [24].

NAFLD treatments focusing on the gut microbiota

NAFLD has turned out to be one among the major contributors to hepatic damage in recent years. If NAFLD worsens, it can lead to a condition known as nonalcoholic steatohepatitis or NASH. NASH can cause liver cancer, fibrosis (excessive accumulation of scar tissue or fibrous tissue in the liver), cirrhosis (advanced stage of liver disease characterized by widespread fibrosis and the formation of regenerative nodules in the liver tissue), and finally liver failure. Thus, a significant risk factor for liver cancer is NAFLD. Primarily dietary changes and lifestyle modifications are currently available treatments for NAFLD, which are conceptually straightforward but extremely challenging to follow. Other pharmacological therapies now in use are aimed at relieving the symptoms of NAFLD rather than addressing the disease’s pathophysiology. Therefore, it is crucial to focus on the underlying mechanisms causing NAFLD development and to pinpoint molecular targets for more effective therapy methods [25]. An attention has been drawn to therapies that target the gut microbiota as possible therapeutic approaches for NAFLD. Here are a few instances of interventions that try to alter the gut flora and how they affect NAFLD [26, 27].

Diet

Fat-rich diets and cholesterol have been linked with liver fibrosis, inflammation, and hepatic steatosis. A 5% weight fall may help with steatosis, but a 7–10% loss is essential to reduce inflammation and fibrosis. It is typically advised to eat a low-fat, low-carb diet with a reduction in calorie intake (500–1000 kcal/day loss to result in a weight loss of 0.5–1.0 kg/week) [28].

Chronic high fat diet treatment to mice was found to be associated with comparatively greater Firmicutes levels and lower Bacterioidetes levels, which causes an elevated F/B ratio. As opposed to that, a diet rich in fiber connected with an elevated level of Akkermansia muciniphila and has been reported to be favorable for reduction of hepatic inflammation [29].

It has been shown that caffeine intake can prevent both the onset of NAFLD and its development to the liver fibrosis stage [30]. Additionally, it has been demonstrated that in a dose-dependent fashion, consumption of coffee is related with improved hepatic enzymes, a lower consequence of cirrhosis and HCC, and fatalities [31]. Additionally, greater coffee consumption has a connection to modifications in the makeup of the gut microflora; in actual, elevated numbers of Prevotella, Bacteroides, and Porphyromonas have been observed in individuals with high coffee intake [32, 33]. Improvements in various obesity markers have been linked to the use of green tea extract, likely as a result of the Bacteroides-to-Prevotella ratio and the F/B ratio returning to normal [34]. It is interesting that after 12 weeks of dosing, people with NAFLD who received a combination of extract of green tea and 2.5% caffeine reported notable changes in their liver enzymes. Additionally, drinking green tea in fluid form has been shown to change the gut flora in numerous analyses [35].

Exercise

Workout, including both cardio and resistance workouts, has been reported to prevent or sometimes reverse NAFLD, making it one of the more promising recommended lifestyle changes. Inactive lifestyle has been linked to the advancement of the condition; hence, it is indicated that exercise is beneficial due to weight loss as well as additional metabolic effects that come along with exercising [36]. Additionally, modifications in the gut microbiota composition as an outcome of workout have been observed. More precisely, exercise has been linked to lower levels of Parabacteroides, Flavobacterium, and Alkaliphilus in animal studies [37].

Further, exercise has been linked to a relative drop in the F/B ratio and a rise in the levels of Verrucomicrobia and Proteobacteria in overweight women [38]. The interaction between the gut and liver may be able to throw light on some of the strong outcomes of exercise in NAFLD patients. For instance, it has been demonstrated that exercise increases the amount of SCFAs, particularly butyrate. Diet alone has less positive impact than exercise. Significantly, exercise has been found to improve sensitivity to insulin and leads to better LDL drop in animal models with high fat diet, primarily because of changes that exercise induces in the microbiome [39]. Interestingly, Barton et al. [40] found that athletes with active workout regimens had higher levels of Akkermansia spp. than those who were more sedentary. Also, Allen et al. [41] found that exercise increased the amount of Faecalibacterium spp. in lean people in comparison with those suffering from obesity. Faecalibacterium and Akkermansia are both evident for having favorable impacts on health.

Prebiotics

Prebiotics are nondigestible fibers that supply nutrition to the good bacteria in the gut. They promote a healthy gut microflora by specifically promoting the growth and function of particular bacterial populations. Prebiotics aid in gut dysbiosis regulation by promoting beneficial bacteria like Bifidobacteria and Lactobacilli, increasing SCFA production for anti-inflammatory effects, strengthening the gut barrier, and modulating the immune response, fostering defense and reducing inflammation [42, 43].

Akbarzadeh et al. [44] performed a placebo-controlled, double-blind, randomized clinical investigation on 80 obese NAFLD individuals, age ranging from 18 to 77 years to investigate the effect of psyllium and ground wheat on physical parameters and liver enzymes. After 10 weeks of treatment, prebiotic supplementation considerably decreased liver levels of ALT and AST as well as BMI compared to placebo.

Probiotics

Probiotics, according to the WHO, are “live microbes that, when given in sufficient quantities, impart beneficial effects on host’s health”. While the usefulness of probiotics is still been proven in a few conditions, their intake is gaining more popularity as a clinical strategy of disease prevention and improved well-being [45].

Typically, probiotics are types of bacteria or yeast that exist naturally in the gut or have favorable impacts on gut health. Probiotics have been proven to help NAFLD patients with their lipid profiles and liver function tests, although the research on liver histologic abnormalities is yet preliminary. Probiotic delivery studies have been challenged by dietary variables that affect the gut flora’s composition as well as by varied formulas and dosages. Probiotics combat intestinal dysbiosis by restoring microbial balance, strengthening the gut barrier, regulating the immune system, and influencing metabolic processes. They colonize the gut, outcompete pathogens, and produce antimicrobial substances, collectively aiding in gut health and reducing inflammation associated with dysbiosis [46, 47].

Although many probiotic strains were investigated for the treatment of NAFLD, many of these treatments involved combination of Bifidobacteria and Lactobacilli. However, next-generation probiotics with promising outcomes have been found, including Faecalibacterium prausnitzii, Akkermansia muciniphila, and Clostridia strains [48].

Numerous findings have stated the impact of probiotics in NAFLD individuals up to this point (Table 2). Improvements in a number of biochemical markers, such as ALT, AST, and TNF, have been noted in the research.

Table 2 Probiotics for NAFLD treatment: human trials
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n sample size, DB-RCT double-blind randomized controlled trial, OL-RCT open label randomized controlled trial, CC HCV-related cirrhosis, RCT randomized controlled trial, DB-PCT double-blind, placebo-controlled trial, NB-RCT non-blinded randomized clinical trial, AC alcohol-related cirrhosis, BP-NAFLD biopsy-proven NAFLD, HP-NASH histology-proven NASH, CH chronic hepatitis linked to HCV.

Synbiotic

Synbiotics, which is a mixture of prebiotics and probiotics, can be consumed to restore a healthy gut flora. Synbiotics have drawn interest as a possible treatment method for the control of NAFLD due to their advantageous effects of encouraging the growth and activity of beneficial bacteria [64].

Synbiotics offer synergistic benefits in treating NAFLD. They reduce hepatic steatosis by improving gut barrier function, control inflammation, and enhance metabolic parameters like insulin resistance and lipid profiles. These effects are attributed to their influence on the gut microbiota and production of beneficial metabolites, including SCFAs, ultimately reducing inflammation in the liver [24].

Scorletti et al. executed double-blind, placebo-controlled phase 2 trial on 104 individuals with NAFLD to observe the impact of synbiotic treatment on hepatic fat content, liver fibrosis-biomarker scores, and gut microbiota composition. The study evaluated the synbiotic comprising of fructo-oligosaccharides and B. animalis subsp. lactis BB-12 against a placebo during a 12-month period. The constitution of the gut microbiota changed as a consequence of the synbiotic therapy, though neither the liver fat content nor the markers of liver fibrosis in NAFLD patients significantly improved. The report emphasizes the need for additional research to investigate alternate NAFLD management techniques [65].

Neyrinck et al. in a study examined the consequences of the symbiotic fructo-oligosaccharides (FOS) and B. animalis combination in middle-aged individuals for a period of 30 days. Comparing the synbiotic therapy to a placebo, it was found that the consistency and frequency of stool was improved while reducing abdominal discomfort. The synbiotic treatment markedly reduced plasma levels of proinflammatory cytokines while having no effect on mood dimensions or the overall composition of the gut microflora. These results indicate that the synbiotic approach may be useful in lowering inflammation and enhancing gut-related symptoms in middle-aged people [66].

Antibiotics

Research and debate about the effectiveness of antibiotics therapy for NAFLD are going on. Antibiotics are often used to treat bacterial infections, but they may also be used to treat NAFLD because it is thought that modifications in the gut microflora may influence disease’s onset and development. Antibiotics can alter the gut microflora by lowering the population of particular bacterial species. Many studies have examined how antibiotics affect NAFLD both in humans and in animals. Reports on animals have demonstrated that taking antibiotics can reduce inflammation and hepatic steatosis (the buildup of fat in the liver). It is challenging to draw firm findings because different research utilizes different antibiotics and treatment lengths [67].

Neomycin, metronidazole, rifaximin, and polymyxin B have been widely employed in the treatment of cirrhosis. Furthermore, the concurrent administration of polymyxin B and neomycin has shown promise in preventing liver lipid accumulation through modulation of the gut microbiota. It is significant to highlight that there are currently no defined recommendations of antibiotics therapy for NAFLD and that their use is still regarded as experimental. The ideal antibiotic regimen, duration, and patient selection criteria must be determined through additional research. Additionally, it is vital to thoroughly assess the possible hazards and long-term implications of antibiotic therapy in NAFLD [68].

Fecal microbiota transplantation (FMT)

Fecal microbiota transplantation (FMT) consists of relocating the fecal microbiota of a healthy donor into the digestive system of a receiver. It has drawn interest as a possible cure for a number of ailments, including NAFLD [69].

Vrieze et al. in their study, investigated how FMT from efficient donors affected metabolic markers in people with metabolic disorder, including NAFLD. The findings demonstrated that FMT with lean donors enhanced insulin sensitivity and decreased liver fat in the receivers. This shows that altering the gut microflora by FMT may be beneficial for treating NAFLD. Individuals with mild hepatic encephalopathy (MHE), a liver disease consequence that may be connected to NAFLD, were estimated for the impacts of FMT on hepatic and cognitive outcomes by Bajaj et al. According to the findings, FMT from a healthy donor significantly improved cognitive function and liver function in recipients in comparison to the placebo group. These findings suggest that FMT may have therapeutic potential for liver-related diseases like NAFLD [70].

Despite the fact that FMT has promise as a viable treatment for NAFLD, further research is necessary to establish its ideal procedures, long-term security, and efficiency. To assess its effectiveness, identify any potential side effects, and develop recommendations for its use in the management of NAFLD, extensive clinical trials are required.

Difficulties in developing NAFLD treatments based on gut microbiota

Customizing treatments based on individual microbial profiles proves challenging and may necessitate personalized strategies. Establishing a clear cause-and-effect relationship between microbiota changes and NAFLD improvement is challenging, necessitating extensive research to unravel the exact mechanisms through which altered microbiota impact liver health. Clinical trials focused on gut microbiota treatments for NAFLD are still in their early stages, lacking robust, large-scale studies that could definitively determine treatment efficacy and safety. The diversity of potential interventions, including prebiotics, probiotics, and fecal microbiota transplantation, demands standardized protocols for dosages, administration, and durations to ensure consistent outcomes.

Navigating complex regulatory pathways for novel microbiota-focused treatments poses challenges in demonstrating safety, efficacy, and regulatory compliance. Successful implementation of microbiota-based interventions often hinges on changes in dietary habits or lifestyles, presenting difficulties in ensuring patient adherence that can influence treatment outcomes. Responses to microbiota-focused therapies can vary significantly among individuals, with some experiencing significant improvements while others see limited or no benefits. The potent influence of placebo effects, especially in studies involving subjective health measures like gastrointestinal symptoms, can confound the interpretation of treatment results. Developing and deploying microbiota-focused treatments can be resource-intensive, potentially limiting accessibility to a broader patient population. Ethical considerations surround interventions like fecal microbiota transplantation, spanning donor screening, informed consent, and potential risks to both donors and recipients [24].

Strategic foresight in studying gut dysbiosis and NAFLD

Future research is likely to focus on developing personalized approaches for diagnosing and treating NAFLD based on an individual’s gut microbiome profile. The development of microbiome-based therapies, including FMT, will be a significant area of research. Identifying specific microbial biomarkers for NAFLD could enable early detection and intervention. Forthcoming studies may also focus on developing non-invasive tests that rely on gut microbiota composition for diagnosis in early stage of the disease. Pharmaceutical companies are likely to invest in the development of drugs that target the gut microbiome to manage NAFLD. Lifestyle modifications, including diet and exercise, will continue to play a crucial role in managing the disease. Futuristic strategies may refine dietary guidelines and exercise recommendations tailored to an individual’s microbiome. However, prospective studies will be essential in assessing the long-term effects of gut dysbiosis on NAFLD.

Public health initiatives such as community education, promoting dietary diversity, and optimizing early-life microbiome development should be incorporated to prevent NAFLD and obesity. Monitoring patients over extended periods will provide insights into the progression of the disease and the efficacy of microbiome-based interventions. Integrating gut microbiome data into the framework of precision medicine will allow for more comprehensive, patient-centered approaches for its management. Genomic, metabolic, and microbial information can be combined to provide a holistic view of an individual’s health. Microalgae extracts may have hepatoprotective properties, which could be explored for its potential in managing NAFLD. Sayuti et al. explored the potential benefits of fucoxanthin, a marine carotenoid, in combating NAFLD by examining its hepatoprotective, anti-obesity, anti-tumor, anti-diabetes, antioxidant, and anti-inflammatory properties. Through analysis of human clinical trials, animal experiments, and in vitro studies, fucoxanthin demonstrated positive effects on lipid metabolism, lipogenesis, fatty acid oxidation, adipogenesis, and oxidative stress, highlighting its therapeutic potential for NAFLD [71]. The coming decade will witness the investigation of the synergistic effects of microalgae in combination with other dietary interventions, probiotics, or medications to address both gut dysbiosis and NAFLD simultaneously.

As researchers continue to unravel the intricate interplay between the gut microbiome and NAFLD, we can anticipate innovative diagnostic methods, personalized treatment strategies, and novel therapeutic interventions that will significantly impact the prevention and management of NAFLD. However, it is important to remain vigilant and ensure that these advancements are ethically and responsibly applied in clinical practice.

Conclusion

This article provides valuable insights into the connection between gut dysbiosis and NAFLD, shedding light on the role of specific bacterial species in NAFLD progression and its associated complications. It reviews clinical studies that investigate this relationship, offering a comprehensive overview of the current state of research. These findings serve as a foundation for future research in the field of gut dysbiosis and NAFLD. Researchers can use this information to design more targeted studies, exploring the mechanisms behind gut microbiota alterations and their role in NAFLD progression. Moreover, the review of various treatments targeting the gut microbiota offers a comprehensive overview of potential interventions, paving the way for further investigations into their long-term safety and efficacy. Ultimately, this article contributes to the growing body of knowledge on gut dysbiosis and NAFLD, guiding researchers toward developing more effective strategies for managing and treating this prevalent liver condition in the future. To optimize the therapy for the treatment of NAFLD, however, future research should concentrate on precision medicine, long-term sustainability of interventions, personalized dietary regimens, and interdisciplinary collaboration.

Availability of data and materials

The data that supports the findings of this study are openly available in the dataset like PubMed, Web of Science, Embase, ScienceDirect, and Google Scholar.

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Acknowledgements

The authors would like to thank Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, India, for providing the necessary infrastructural facilities to carry out the work.

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  1. Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, 411 018, India

    Pranali A. Jadhav, Asha B. Thomas, Rabindra K. Nanda & Sohan S. Chitlange

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  2. Asha B. Thomas
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  3. Rabindra K. Nanda
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  4. Sohan S. Chitlange
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Idea: Asha Thomas; study design: Pranali Jadhav and Asha Thomas; data acquisition: Asha Thomas and Pranali Jadhav; data analysis: Pranali Jadhav and Sohan Chitlange; data interpretation: Asha Thomas and Rabindra Nanda; drafting the manuscript: Pranali Jadhav and Asha Thomas; manuscript revision: Asha Thomas, Rabindra Nanda, and Sohan Chitlange

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Jadhav, P.A., Thomas, A.B., Nanda, R.K. et al. Correlation of non-alcoholic fatty liver disease and gut microflora: clinical reports and treatment options. Egypt Liver Journal 14, 21 (2024). https://doi.org/10.1186/s43066-024-00327-6

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