The most common potential life-threatening complications in patients with cirrhosis and ascites is SBP where the mortality rate is alternating between 30 and 50%; hence, the prompt diagnosis and treatment are the most common variable causes in reducing morbidity and mortality of SBP. Bacterial translocation is considered the pathophysiological hallmark in developing spontaneous bacterial peritonitis (SBP). Prokinetics can increase GIT motility, decrease small bowel transit time, decrease bacterial translocation and the possibility of SBP.
Our study was conducted for evaluating the effectiveness and safety of itopride, as a prokinetic drug, in cirrhotic patients with spontaneous bacterial peritonitis to detect its role in secondary prophylaxis of SBP.
The major strength of this study is that it shed a light about the role of prokinetics in prevention of SBP in cirrhotic ascitic cases through its effect on decreasing the probability of a bacterial overgrowth via increasing the intestinal motility.
In our study, we enrolled 80 cirrhotic ascitic cases with a previous event of SBP who were classified into two groups, each of 40 cases. SBP was common in males in the two studied groups (21 and 25 patients respectively). The mean age was 57.50 ± 6.48 years in group I and 59.40 ± 6.16 years in group II without any a significant difference in both studied groups in age and sex distribution. The two groups were mostly cross-matched.
In this study, the most common symptoms of SBP which presented in both groups were abdominal pain in 39 patients (97.5%) in group I but in 37 cases (92.5%) in group II and fever presented in 31 patients (77.5%) through group I but 34 cases (85%) in group II then vomiting and hepatic encephalopathy (22.5% and 20% respectively) of group I and 40% and 22.5% respectively of group II without a significant differences in both studied groups as regards the clinical manifestations plus laboratory characteristics of our studied patients. This shows that the patients of both studied groups are cross matched.
In the current work, the degree of ascites in SBP patients was either moderate up or marked. Moderate ascites presented in 32 cases 80% (group I) while (group II) the moderate ascites was detected in 17 cases (42%), while marked ascites was reported in 20% and 57.5% of group I and II respectively with no statistically significant difference in group I and group II. These results corroborate the ideas of [6] who stated that ascitic fluid infection mostly developed when the ascitic fluid volume was at its maximum.
In the current study, patient groups showed low serum albumin level but high serum bilirubin, INR, AST, and ALT levels which mean the most reported cases of that disease (SBP) were Child-Pugh class C in both groups without a statistically significant variance in the studied groups [7].
Diagnostic paracentesis is the main stay in SBP diagnosis and can differ in SBP from secondary peritonitis [8]. It was performed to all cases in groups I and II included in this study. There was no statistically significant variance in the two patient groups according to the ascitic fluid analysis.
The mean value of serum-ascites albumin gradient (SAAG) in our current study was > 1.1 g/dl in both patient groups which confirmed that the etiology of ascites was portal hypertension in our SBP patients. Agarwal et al. [9] stated that SAAG levels of > 1.1 g/dl confirms that ascites is because of portal hypertension regardless the infection.
As regards to the ascitic fluid culture, the most reported cases of SBP, in both groups, were positive for gram-negative cocci without a statistical significant difference between both groups. These results did not match with Mostafa et al. [10] who found that the majority of their cases of SBP because of gram-positive cocci. In our study, we found that the most common and virulent organisms isolated from the cases were Escherichia coli species and Klebesiella but the less common was Staph-Gram-positive cocci, this matched with Novella et al. [11] who observed that of E. coli was isolated in 90% of their SBP patients.
In our study, on follow-up of the studied SBP patients, we observed, at the second month, a total of 14 episodes (35%) of recurrent SBP infections in group II (norfloxacin only) versus 5 episodes (12.5%) in group I (norfloxacin plus itopride) with an apparent statistically significant difference. The same variance was also detected in both studied groups in that fourth and sixth month follow-up; however, this difference was not significant. So the usage of both norfloxacin plus itopride (group I ) showed a better SBP incidence free through the study follow-up period being 87.5%, 89.5, and 87.5% on the follow-up periods at second, fourth, and sixth month respectively, as compared to that, on usage of norfloxacin only as a SBP secondary prophylactic measure in group II. This accompanied with death of 20% of group II cases compared to death of only 5% of group I cases through the fourth and sixth months follow-up.
According to the present results, regarding the percentage of patients in group I (under treatment with norfloxacin plus itopride) who developed recurrent SBP which represented 12.5%, 10.5%, and 12.5% of cases through the follow-up period at the second, fourth, and sixth months respectively. As a probable explanation for the developing of SBP by the translocation of the bacteria from the intra (intestine) to extra (ascitic fluid) could not be the only route of infection, and the presence of ascitic bacterial DNA can support that condition [12].
In the present study, we reported a 65%, 73.5%, and 71.9% of SBP incidence free through the second, fourth, and sixth months follow-up period respectively in group II who received norfloxacin only. This result matched with Ghafar et al. [13] study that evaluated norfloxacin use in prophylaxis of SBP that established 40% decreasing in the frequency of SBP, among their patients. Also, Hanouneh et al. [14] found 72% prevention rates of SBP in their study.
On the other hand, Fernandez et al.’s [15] work showed that 93% reducing in the SBP incidence cases, who received norfloxacin. This difference might be due to the type of patients included in our study or the prophylaxis of SBP with norfloxacin only becomes less active as before, because of increasing the incidence of quinolones bacterial resistance in cirrhotic patients flora in the stool due to over use of these drugs [16].
In the current study, the median time for recurrent SBP development was a highly significant prolonged in group I when we compared it by the group II. This was associated with a statistically significant improvement in patient survival rate. The recurrent SBP development rate was significantly decreased in group I (5%) when we compared that by the other group (II) (20%) and that difference in the incidence of SBP recurrence occurred in only in the first 2 months of follow-up due to the number of patient’s death increased through the last 4 months that was mainly due to recurrent SBP (15%), HRS (12.5%), and hepatic encephalopathy (7.5%) in group II. While only HRS was reported as the only cause of death in 5% of group I. These results highlight the advantage of using the prokinetics agents (itopride) in combination with norfloxacin in SBP secondary prophylaxis.
The most common causes of mortality allover our study were hepatic encephalopathy, sepsis, and hepatorenal syndrome. And the results showed the beneficial effect of prokinetices (itopride) on group I cases that death in this group was 5% only compared to 20% death rate in group II.
On the same hand, PC Revaiah et al. [17] reported that combined use of prokinetics drugs could considerably reduce the possibility of small intestinal bacterial over growth because of increasing the intestinal motility, which lead to reduce bacterial translocation, the path of bacteria from the intra (intestine) to extra-intestinal such as ascitic fluid and this condition can be useful in SBP prophylaxis and treatment approach.
On other hand, Frazee et al. [18] found that long-term antimicrobial prophylaxis may be useful and has a benefit in cirrhotic patients. However, the magnitude of the benefits was less and the frequency of overall infections and mortality did not change [19].
In the current study, we did not report any side effects from usage of itopride which supported by Huang X (2012), [20] who found that itopride was the least prokinetics agent side effects through the incidence rate of its side effects. Also, itopride had an excitatory influence on the small and large intestine better than cisapride or mosapride [21].
The main limitation of this study is being a single center study with a problem of generalizing the findings. Therefore, larger multicenter studies are crucially needed to document the role of prokinetic drugs in prevention of SBP.