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Egyptian Liver Journal

Table 1 Group-specific primers based on 16 S rDNA sequences

From: Can probiotics play a role in Helicobacter pylori (H. Pylori) eradication?

Target bacteria

Primer

Sequence (5′–3′)

PCR product size

Bifidobacterium PCR assay

F-bifido

CGCGTCYGGTGTGAAAG

 

R-bifido

CCCCACATCCAGCATCCA

244 pb

MGB-bifido

AACAGGATTAGATACCC

 

Lactobacillus PCR assay

F-lacto

GAGGCAGCAGTAGGGAATCTTC

 

R-lacto

GGCCAGTTACTACCTCTATCCTTCTTC

126 pb

MGB-lacto

ATGGAGCAACGCCGC

 
  1. Strain (Bifidobacterium longum and Lactobacillus acidophilus) Quantitative PCR assay based on genus-specific 16S rDNA primers and 3′ minor groove binder (MGB) probes for accurate detection and quantification of a wide range of Bifidobacterium spp. (30 species) and Lactobacillus spp. (15 species) in fecal samples
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