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Table 5 Comparison between the two cholestatic groups (BA & non-BA) and control regarding primary conjugated bile acids in serum and urine (μmol/L)

From: The value of blood and urine metabolomics in differential diagnosis of cholestasis in infants

Primary conjugated bile acids (μmol/L)

Biliary atresia (n = 37)

Non-biliary atresia (n = 65)

Control (n = 102)

H

p

Serum

Glycholic acid (GCA)

 Min. – Max.

0.80 – 144.0

0.0 – 281.0

0.0 – 3.0

126.824*

<0.001*

 Median (IQR)

22.9(14.60 – 44.0)

18.0 (6.60 – 40.30)

0.10ab (0.02-0.70)

Glychochenodeoxycholic acid (GCDCA)

 Min. – Max.

6.80 – 163.0

0.02 – 167.0

0.0 – 2.50

130.766*

<0.001*

 Median (IQR)

42 (30.20 – 66.30)

22.80a (12.2 -52.0)

0.08ab (0.04 -0.60)

Taurochlolic acid (TCA)

 Min. – Max.

0.12 – 155.70

0.0 – 65.90

0.0 – 0.86

113.598*

<0.001*

 Median (IQR)

24.7 (12.80 -39.8)

17.8a (4.2 -32.9)

0.06ab (0.01 –0.21)

Taurochenodeoxycholic acid (TCDCA)

 Min. – Max.

0.13 – 183.0

0.0 – 208.0

0.0 – 1.30

113.738*

<0.001*

 Median (IQR)

45.0 (36.90 –68.60)

20.5a (9.0 – 45.50)

0.03ab (0.0 – 0.50)

TCDCA/CDCD ratio

 Min. – Max.

13.0 – 11920.0

0.0 – 20800.0

0.40 – 100.0

116.004*

<0.001*

 Median (IQR)

686.0(260.0– 2740)

683.33(91.4 -2420)

9.0ab (3.58 – 20.0)

GCDCA/CDCA ratio

 Min. – Max.

230.0 – 16300.0

0.25 – 16700.0

0.50 – 600.0

124.972*

<0.001*

 Median (IQR)

3860.0(2240 -6300)

1720.0a (404.2-3640)

22.50ab (8.0 –85.0)

Urinary

Glycholic acid (GCA)

 Min. – Max.

0.0 – 68.0

0.0 – 64.50

0.0 – 3.0

101.57*

<0.001*

 Median (IQR)

6.60 (3.10 – 18.0)

6.30(1.40– 12.50)

0.10ab (0.02 –0.70)

Glychochenodeoxycholic acid (GCDCA)

 Min. – Max.

0.0 – 67.0

0.0 – 52.0

0.0 – 2.50

36.142*

<0.001*

 Median (IQR)

8.60 (0.30 – 17.0)

0.60 (0.15 – 7.40)

0.08ab (0.04 – 0.60)

Taurochlolic acid (TCA)

 Min. – Max.

0.0 – 83.80

0.0 – 33.0

0.0 – 0.86

103.752*

<0.001*

 Median (IQR)

5.60 (2.40– 17.40)

2.60 (0.44 – 9.50)

0.06ab (0.01 –0.21)

Taurochenodeoxycholic acid (TCDCA)

 Min. – Max.

0.0 – 56.70

0.0 – 65.60

0.0 – 1.30

55.941*

<0.001*

 Median (IQR)

1.20 (0.20 – 17.0)

0.60 (0.17 – 6.30)

0.03ab (0.0 – 0.50)

  1. IQR Interquartile range, SD Standard deviation
  2. H H for Kruskal-Wallis test, pairwise comparison bet. 2 groups was done using post hoc test (Dunn’s for multiple comparisons test)
  3. p p value for comparing the studied groups
  4. *Statistically significant at p ≤ 0.05
  5. aStatistically significant with the biliary atresia group
  6. bStatistically significant with the non-biliary atresia group