Table 1 PCR primers sequences of the genes analyzed
Sr. no. | Gene | Forward primer | Reverse primer |
|---|---|---|---|
1 | GAPDH | 5′-TGTGAACGGATTTGGCCGTA-3′ | 5′-ACTGTGCCGTTGAATTTGCC-3′ |
2 | TLR-4 | 5′-TTTGCTGGGGCTCATTCACT-3′ | 5′-GACTCGGCACTTAGCACTGT-3′ |
3 | LBP | 5′-TGTTACCACATGACTCCGGC-3′ | 5′-AGGTGGGCAGGATCACAAAG-3′ |
4 | NRF2 | 5′-CGAGATATACGCAGGAGAGGTAAGA-3′ | 5′-GCTCGACAATGTTCTCCAGCTT-3′ |
5 | HO-1 | 5′-ACATCGACAGCCCCACCAAGTTCAA-3′ | 5′-CTGACGAAGTGACGCCATCTGTGAG-3′ |
6 | TNF-α | 5′-GTGGAACTGGCAGAAGAG-′ | 5′-AATGAGAAGAGGCTGAGAC-3′ |
7 | IL-6 | 5′-TGGATGCTACCAAACTGGAT-3′ | 5′-CCTCAAAGCCAAGATGAGAA-3′ |
8 | IL-4 | 5′-GTAGGGCTTCCAAGGTGCTT-3′ | 5′-GGCATCGAAAAGCCCGAAAG-3′ |
9 | IL-10 | 5′-AAGGGTTACTTGGGTTGCCA-3′ | 5′-TTCAGCTTCTCACCCAGGGA-3′ |
10 | HGF | 5′-TGAGTTATGTGCTGGGGCTG-3′ | 5′-CACATCCACGACCAGGAACA-3′ |
11 | CAT | 5′-CTGGATGGATTCTCCCCCGC-3′ | ′-TCAGGAAACGGCATCAAAAGC-3′ |
12 | GSS | 5′-AACGAGCGAGTTGGGATG-3′ | 5′-TATGTCACCACGTCGGAGGA-3′ |
13 | SOD | 5′-TTGGCCTGTGGAGTGATTGG-3′ | 5′-AGCCCAGTCAAAGGAGTCAC-3′ |