Table 1 Thermal cycler condition of insulin receptor substrate-1 G972R (rs 1801278)
| Phases | Cycle number | Temperature (°C) | Time |
|---|---|---|---|
| Initial denaturation | 1 | 95 | 1 min |
| Amplification | |||
| • Denaturation | 35 | 95 | 1 min |
| • Annealing | 61 | 45 s | |
| • Extension | 72 | 1 min | |
| Final extension | 1 | 72 | 7 min |