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Egyptian Liver Journal

Table 1 Thermal cycler condition of insulin receptor substrate-1 G972R (rs 1801278)

From: Insulin receptor substrate-1 G972R single nucleotide polymorphism in Egyptian patients with chronic hepatitis C virus infection and type 2 diabetes mellitus

Phases

Cycle number

Temperature (°C)

Time

Initial denaturation

1

95

1 min

Amplification

 • Denaturation

35

95

1 min

 • Annealing

 

61

45 s

 • Extension

 

72

1 min

Final extension

1

72

7 min

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