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Table 1 Thermal cycler condition of insulin receptor substrate-1 G972R (rs 1801278)

From: Insulin receptor substrate-1 G972R single nucleotide polymorphism in Egyptian patients with chronic hepatitis C virus infection and type 2 diabetes mellitus

Phases Cycle number Temperature (°C) Time
Initial denaturation 1 95 1 min
Amplification
 • Denaturation 35 95 1 min
 • Annealing   61 45 s
 • Extension   72 1 min
Final extension 1 72 7 min