Table 1 qPCR reaction mix
PCR reaction mix components | Volume (μL)/20-μL Reaction | Final concentration |
|---|---|---|
TaqMan universal master mix II, with no UNG, 2× | 10.0 | 1× |
TaqMan MicroRNA Assay (20×) | 1.0 | 1× |
cDNA/DNA template + RNase free water | 9.0 | 1 to 100 ng |
Total volume | 20.0 | – |