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Table 1 qPCR reaction mix

From: Role of circulating miRNA-17-3P as a potential diagnostic biomarker for sporadic colon cancer in Egyptian cohort

PCR reaction mix componentsVolume (μL)/20-μL ReactionFinal concentration
TaqMan universal master mix II, with no UNG, 2×10.0
TaqMan MicroRNA Assay (20×)1.0
cDNA/DNA template + RNase free water9.01 to 100 ng
Total volume20.0