From: CTNNB1 polymorphism (rs121913407) in circulating tumor DNA (ctDNA) in Egyptian hepatocellular carcinoma patients
Component
Volume
TaqMan genotyping Master mix (2×)
10 μL
TaqMan SNP genotyping assay (primers and probes) (40×)
0.5 μL
Extracted DNA
Equivalent to 20 ng
DNase-free water
To final volume 20 μL
Total volume per well
20 μL