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Table 2 PCR reaction mix in each sample

From: CTNNB1 polymorphism (rs121913407) in circulating tumor DNA (ctDNA) in Egyptian hepatocellular carcinoma patients

Component

Volume

TaqMan genotyping Master mix (2×)

10 μL

TaqMan SNP genotyping assay (primers and probes) (40×)

0.5 μL

Extracted DNA

Equivalent to 20 ng

DNase-free water

To final volume 20 μL

Total volume per well

20 μL