Fig. 4
From: Characterization of mesenchymal stem cells isolated from Wharton’s jelly of the human umbilical cord
Expression of surface marker of the isolated UCWJ-MSCs. A Flow cytometry analysis of the surface molecule markers. Positive expression of mesenchymal stem cell markers (CD44, CD90, and CD105) and negative hematopoietic markers (CD34 and CD45) in WJ-MSC at passage 4. B Thidium bromide 2% stained agarose gel electrophoresis for amplified CD44, CD90, and CD105 expressed by MSCs at P2, P4, and P6. Lane1 DNA ladder, lanes (2–4) CD44 amplicons of 232 bp, lanes (5–7) CD105 amplicons of 254 bp, lanes (8–10) CD90 amplicons of 233 bp. GAPDH amplicon of 498bp was used as an internal control for CD expression. C Ethidium bromide 2% stained agarose gel electrophoresis for amplified CD34 expressed by MSCs at P 2, P4, and P6. Lane1 DNA ladder, lane 2, -ve con, lane 3, +ve con. Lane (4–6) CD34 amplicons of P2, P4, and P6. GAPDH amplicon of 498bp was used as an internal control