The expression of microRNA-331-3p and microRNA-23b3 in Egyptian patients with early-stage hepatocellular carcinoma in hepatitis C-related liver cirrhosis

Aboelwafa, Reham A.; Ellakany, Walid Ismail; Gamaleldin, Marwa A.; Saad, Marwa A.

doi:10.1186/s43066-021-00122-7

Egyptian Liver Journal

Table 1 Comparison between the three studied groups according to the demographic data, laboratory investigations, and serum concentrations of miR-331-3p and miR-23b-3p

From: The expression of microRNA-331-3p and microRNA-23b3 in Egyptian patients with early-stage hepatocellular carcinoma in hepatitis C-related liver cirrhosis

	Group I (HCV-related liver cirrhosis) (n = 100)	Group II (HCC group) (n = 50) (16 stage 0 BCLC, 34 stage A BCLC)	Group III (healthy controls) (n = 50)	Test of Sig.	p
Age (years)
Mean ± SD	47.4 ± 9.7	50.6 ± 5.2	47.5 ± 9.3	F = 2.489	0.086
Median (min.–max.)	49(25–61)	51(41–59)	48(32–63)	F = 2.489	0.086
Gender
Male	79 (79%)	40 (80%)	35 (70%)	χ² = 1.863	0.394
Female	21 (21%)	10 (20%)	15 (30%)	χ² = 1.863	0.394
ALT (IU/L)
Mean ± SD	59.7 ± 35.1	60.4 ± 26.3	19.0 ± 7.7	H = 72.496^*	< 0.001^*
Median (min.–max.)	56.5^a (8.4–165)	48^a (33–127)	18.4^b (5.8–38.1)	H = 72.496^*	< 0.001^*
AST (IU/L)
Mean ± SD	53.2 ± 30.1	55.3 ± 24.8	18.7 ± 6.3	H = 84.833^*	< 0.001^*
Median (min.–max.)	45.5^a (6.9–155)	44.5^a (30–119)	17.6^b (6.4–30.2)	H = 84.833^*	< 0.001^*
Total bilirubin (mg/dl)
Mean ± SD	1.2 ± 0.6	1.1 ± 0.4	0.7 ± 0.3	H = 25.660^*	< 0.001^*
Median (min.–max.)	1.1^a (0.2–2.8)	1^a (0.4–2)	0.8^b (0.1–1.2)	H = 25.660^*	< 0.001^*
Albumin (g/dl)
Mean ± SD	3.9^b ± 3.1	3.6^c ± 2.6	4.3^a ± 4.8	F = 46.804^*	< 0.001^*
Median (min.–max.)	4.0 (3.3–4.5)	3.6 (3.2–4.0)	4.2 (3.4–5.2)	F = 46.804^*	< 0.001^*
Hemoglobin (g/dl)
Mean ± SD	13.6^a ± 1.8	12.2^b ± 1.3	14.1^a ± 1.1	F = 22.005^*	< 0.001^*
Median (min.–max.)	13.9 (8.5–16.4)	12.0 (10.0–15.0)	14.4 (12.1–15.9)	F = 22.005^*	< 0.001^*
Total leukocytic count (× 10⁹)
Mean ± SD	5.2^b ± 1	4.2^c ± 1.1	6.7^a ± 1.8	F = 51.129^*	< 0.001^*
Median (min.–max.)	5.1 (3.5–7.1)	4 (2.4–6.3)	6.5 (4.3–10.2)	F = 51.129^*	< 0.001^*
Platelet count (× 10⁹)
Mean ± SD	160.9^a ± 46.8	130.2^c ± 21.5	223.1^b ± 44.1	F = 67.135^*	< 0.001^*
Median (min.–max.)	158 (63–254)	134.5 (91–162)	238.5 (126–358)	F = 67.135^*	< 0.001^*
AFP (ng/ml)
Mean ± SD	140.3 ± 45.2	183.1 ± 77	1.0 ± 0.4	H = 117.964^*	< 0.001^*
Median (min.–max.)	138^b (1.8–240)	196^a (58–287)	1^c (0.2–1.9)	H = 117.964^*	< 0.001^*
miR-331-3p (2^−∆CT)
Mean ± SD	2.2 ± 1.3	7.6 ± 10	1.1 ± 0.5	H = 69.738^*	< 0.001^*
Median (min.–max.)	1.98^b (0.1–7.8)	3.8^a (0.1–41)	1^c (0.1–3.2)	H = 69.738^*	< 0.001^*
miR-23b-3p (2^−∆CT)
Mean ± SD	0.9 ± 0.8	0.3 ± 0.3	1.5 ± 1.1	H = 54.230^*	< 0.001^*
Median (min.–max.)	0.8^b (− 0.98 to 3.2)	0.2^c (0–1.7)	1.3^a (− 1 to 3.8)	H = 54.230^*	< 0.001^*

χ² chi-square test, F F for ANOVA test, H H for Kruskal-Wallis test, p p-value for comparing between the studied groups
Means/median with common letters are not significant (i.e., means with different letters are significant)
*Statistically significant at p ≤ 0.05

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